BASi Tubing and Connectors

Small bore (120 µm ID) Teflon or PEEK tubing is used to reduce the delay between dialysis and sample collection. The swept volume is ca. 1.2 µL/10 cm. Tubing is sold in 1 m lengths and is connected to 23 ga. syringe needles, dialysis probes, swivels, and cannulae (0.6 - 0.8 mm OD) by means of a flanged plastic connector. The connector "splices" the tubing and cannula together, eliminating dead space between them.

If bacterial contamination is a concern between tubing uses (and it should be if you are working with ACh, amino acids, glucose, lactate, or pyruvate in particular), flush some diluted ProClin150™ (CF-2150) through the tubing between uses. [Note: if a liquid swivel is plumbed in your system, do NOT use a syringe to manually fill the tubing and/or liquid swivel with the ProClin. If you do, you will likely damage your swivel. Instead, use a syringe pump to flush the tubing and swivel with the ProClin at normal microdialysis flow rates, or aspirate the ProClin solution through the system via suction from a syringe.] Flush several volumes of water or perfusate though the system before reusing tubing or swivel flushed with ProClin150.

Ordering Information

 

CF-2150	ProClin150™ Preservative, 100 mL
MD-1510	Tubing Connectors, 20/pkg.
MF-5164	FEP Teflon Tubing, 0.65 mm OD x 0.12 mm ID, 1 meter (clear).
MF-5366	PEEK Tubing, 0.65 mm OD x 0.12 mm ID, 1 meter (tan).
MD-1511	PEEK Tubing, 0.65 mm OD x 0.12 mm ID, 1 meter (blue).
MD-1512	PEEK Tubing, 0.65 mm OD x 0.12 mm ID, 1 meter (red).

 

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