BASi
Bioanalytical System Inc.
BASi is a drug development firm providing contract research services and unique products for the pharmaceutical, biotechnology and medical device industries.
BASi LCEC Flowcells
BASi has developed a variety of flow cells to
fit a variety of applications. How do you make a choice? To begin, the analyte in most cases determines the working electrode
material. For most oxidizable/reducible organic compounds, a glassy carbon electrode is used, for acetylcholine a platinum or “
wired” glassy carbon is used(since enzymatic derivatization yields H2O2 for detection), Hg/Au is used to
determine thiols/disulfides, a copper electrode for carbohydrates, gold for pulsed electrochemical techniques-call BASi and we will
discuss detection of your analyte.
Second, is the column of standard size or is
it microbore? In many instances a cell used for standard chromatography can be optimized for a microbore system by the simple change
of gasket thickness-thus reducing the cell-dead-volume. What flow pattern, cross-flow or radial-flow, will give optimal response, thus
resulting in lower detection limits? Generally radial-flow cells are best suited for microbore systems and cross-flow for standard
systems. If fraction collection or connection to a second detector (UV, fluorescence, or MS) is required, then the cross-flow with
reference port configuration (MF-1092) should be used.
The following sections describe a number of options. Call BASi if it is not clear to you which cell best fits your system/application. A complete flowcell consists of two main parts:
- The Auxiliary Electrode also includes a phenolic base, arms, and exit tube.
- The Cell Kit includes a glassy carbon working electrode, gasket, reference electrodes, the retaining hardware for these electrodes, and a polishing kit.
In many cases several types of Cell Kits are compatible with a particular Auxiliary Electrode.
You can order a COMPLETE flowcell or REPLACEMENT gaskets and working electrodes.
